RT-PCR Testing

Polymerase chain reaction (PCR) is a molecular laboratory technique used to amplify specific segments of DNA for genetic and molecular analysis.

Real-time PCR (RT-PCR or quantitative PCR) is an advanced molecular diagnostic technique in which DNA amplification is monitored continuously during the amplification process using fluorescent detection systems. Compared with conventional PCR methods, real-time PCR allows more rapid and sensitive detection and analysis of amplified genetic material.

Real-time PCR systems generally include a thermal cycler, fluorescence detection system, computerized analysis software, and specialized reagents utilized for amplification and signal detection.

Fluorescent signal detection in real-time PCR may involve different approaches including intercalating fluorescent dyes or probe-based fluorescence systems such as fluorescence resonance energy transfer (FRET)-based methods.

During the amplification process, changes in fluorescence intensity are monitored and analyzed using specialized software to evaluate amplification patterns, threshold cycles, quantification data, and melting curve characteristics depending on the testing protocol.

Real-time PCR offers several advantages including rapid processing, high sensitivity, reduced contamination risk due to closed-tube workflows, quantitative analysis capability, and multiplex testing options in selected applications.

RT-PCR techniques may be utilized in selected reproductive genetics, molecular diagnostics, infectious disease testing, mutation analysis, gene expression studies, pathogen detection, and laboratory evaluation workflows depending on clinical indications.

Real-time PCR testing at Krishna IVF forms part of broader reproductive genetics, molecular diagnostics, laboratory medicine, and individualized reproductive medicine workflows.