Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues, or organisms. These small molecules and their interactions within a biological system are collectively known as the metabolome.

Krishna IVF Clinic’s vision of providing comprehensive, innovative, and state-of-the-art reproductive health services, in a reliable, affordable, and customer-centric manner, Dr. G. A. Rama Raju founded Krishna IVF Clinic Private Limited in the year 1996 and has completed a glorious silver jubilee of its existence, tagged to its great legacy over the two and half decades. Krishna IVF has been extended to diagnostic services to provide Therapeutic Drug Monitoring, Newborn Screening, and Serum Steroidal panel since 2018.

Steroid Panel of Metabolic


Krishna IVF team is using the latest technologies – GERI Time Lapse technology, RT PCR, Sangers sequencing, Liquid Chromatography-Mass Spectrometry (LCMS), VIDAS PC, IT – data management, and quality norms to bring the best to our patients. With a highly experienced and qualified team of Gynaecologists, Embryologists, Geneticists, Pathologists, Biochemists, Consultants, and Paramedical staff. We have several full-fledged departments in the laboratory under one roof, such as Cytogenetics, Molecular genetics, Embryology, Andrology, Biochemistry, and Proteomics.

Steroid Panel:

Multiparametric immunoassay system for medium-throughput VIDAS® is a reliable and easy-to-use automated benchtop immune analyzer. Based on the Enzyme-Linked Fluorescent Assay (ELFA) technology, it provides high-quality on-demand test results. Over 100 parameters are available in a single-test format for routine, complementary, specialized, or emergency testing to quantify hormonal levels -Estradiol, Luteinizing Hormone, Follicular Stimulating Hormone, HCG and Progesterone, etc.

Liquid Chromatography with tandem mass spectrometry (LC-MS-MS) is a powerful Bio-analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry.

Follicular steroid analysis:

Follicular fluid (FF) provides nourishment for oocyte development. Proteins in FF regulate the external as well as internal environment of oocytes. As such, these proteins serve a critical role in fertilization and embryo development.

The goal of study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (FF) samples obtained during oocyte retrieval from women undergoing IVF treatment and to identify physiological pathways associated with the proteins which may help to predict the oocyte quality for selecting the oocyte to be stored for future use.

Sperm Proteomics:

Proteomics, especially sperm proteomics, is a new field in human reproduction studies. Proteomics can elucidate complex biological systems. Spermatozoa contain relatively higher amounts of membrane proteins than other cells; therefore, they are very suitable for proteomic studies. Recently, the application of proteomic approaches such as the two-dimensional polyacrylamide gel electrophoresis, mass spectrometry, and differential in-gel electrophoresis has identified several sperm-specific proteins. These findings have provided a further understanding of protein functions involved in different sperm processes and the differentiation of a normal state from an abnormal one. In addition, studies on the sperm proteome have demonstrated the importance of spermatozoal post translational modifications and their ability to induce physiological changes responsible for fertilization.

Large-scale proteomic studies to identify hundreds to thousands of sperm proteins will eventually lead to the development of novel biomarkers that will help identify fertility and complete contraception; these protein biomarkers may be used to diagnose and treat sperm dysfunction better.

Subtopic: Quantification of targeted functional sperm proteins biomarkers can be done by using the LCMS

Planned project

Reproductive toxicology studies:

Reproductive toxicology studies the adverse effects on the male and female reproductive system from exposure to chemicals or physical agents. These are Phthalates, Bisphenol A, and phthalic acid esters, which are often used as plasticizers for polymers such as polyvinylchloride. They are widely applicable in various products, including personal care goods, cosmetics, paints, printing inks, detergents, coatings, and food packaging. Determination and quantification of the phthalates exposure can be done by analyzing the serum, urine, and semen samples of the infertility patients, and were separated on a reversed-phase column using UPLC coupled tandem quadrupole mass spectrometer.

Spent culture media analysis:

The goal of embryo culture in an assisted reproductive (ART) program is to improve the quality of embryos developing in the laboratory and the chances of successful delivery of a healthy baby. Culture conditions for human embryos have evolved over the past thirty years.

The metabolomics field has recently developed new technologies and methodologies which can perform a quantitative and qualitative analysis of the metabolites produced and secreted by any cell, tissue, or even simple organisms. For example, non-invasive quantitative techniques are used to assess the metabolic status of the embryo by using embryo-spent culture media to predict embryo viability and the likelihood of pregnancy. In addition, research has increasingly focused on the metabolites of spent culture media using Spectroscopic techniques such as Mass Spectrometry.

We keep track of technological advancements and offer access to the latest developments in research. We comply with the most stringent quality and ethical norms to achieve consistent safety and quality. We continuously expand our value-added services to better serve patients with dignity, respect, and compassion.

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