Training In Various Areas Of Human Assisted Reproduction Techniques

Training in Basic infertility:

During this course program each participant receives training to develop knowledge and skills in following areas:
1. Introduction to ovulation
2. Work up-infertility
3. Patient counseling
4. IUI lab procedures
5. IUI Laboratory equipment
6. Freezing protocols for semen and TESE samples
7. Understanding ivf failures
8. Visit to embryology lab
9. Visit to genetics lab
10. Consent forms & documentation

Mode of teaching:
• Lectures and multimedia presentations.
• Interactive discussions.

Each participant will receive:
• Certificate of training

Duration of hands on training: 7-days

Training in Advanced infertility:

During this course program each participant receives training to develop knowledge and skills in following areas:
1. Introduction to ovulation
2. Work up-infertility
3. Patient counseling
4. IUI lab procedures
5. IUI Laboratory equipment
6. Freezing protocols for semen and TESE samples
7. Tubal factor review (HSG films & laparoscopy films)
8. Hysteroscopy
9. Understanding ivf failures
10. Set up of IUI and IVF lab
11. Visit to embryology lab
12. Visit to genetics lab
13. Consent forms & documentation

Mode of teaching:
• Lectures and multimedia presentations.
• Interactive discussions.

Each participant will receive:
• Certificate of training

Duration of hands on training: 14-days

Hands on Training in Andrology Lab Protocols

During this course program each participant receives one-on-one training to develop knowledge and skills in following areas:
• Semen sample collection and delivery.
• Semen analysis.
• Manual and Computer assisted semen analysis (CASA).
• Sperm morphology assessment (WHO and Tygerberg strict criteria).
• Fructose detection in seminal plasma (qualitative)
• Viability testing (Eosin & Nigrosin stain).
• 24 hrs survival test
• Hypo-osmotic swelling test.
• Leukocyte detection (Endtz Test/Peroxidase staining).
• Antisperm antibodies detection (Immunofluorescence test)*
• Media preparation
• Consent forms & documentation
Sperm preparation protocols:
• Preparation of ejaculated sperm for ART:
- Removal of Seminal plasma
- Double density gradient technique
- Swim-up method
• - Concentration method (for low count samples)
• Method of retrieval of sperm from retrograde ejaculate*
• Sperm retrieval and preparation of testicular biopsy specimens (rat or mouse specimens will be used)*
Cryopreservation protocols:
Human spermatozoa freezing:
-By vapour freezing method
- By Planar technique
Isolation and cryopreservation of sperm from testicular biopsy specimens*
Safety guidelines for andrology laboratory.

* Demonstration
Mode of teaching:
• Lectures and multimedia presentations.
• Interactive discussions.

Each participant will receive:
• Course manual.
• Certificate of training

Duration of hands on training: 7-days

Hands on training in Assisted reproduction techniques (IVF/ICSI)

This course provides comprehensive hands on training in the laboratory practices of IVF and ICSI. The syllabus covers the following areas:
Hands-on Laboratory Training:
Culture techniques
• Preparation of culture dishes for IVF/ IVF-ICSI cases
• Embryo culture
• Blastocyst culture
• Preparation of media for Hyaluronidase
• Preparation of media for vitrification of embryos and blastocysts
IVF Techniques
• Pipette pulling and Pipetting techniques
• Ovum pickup from follicular fluid
• Oocyte handling
• Denudation of oocytes for ICSI
• Morphological assessment of oocytes
• IVF procedure
• ICSI: Intracytoplasmic sperm injection
• Assessment of oocyte fertilization
• Embryo handling
• Embryo grading
• Embryo transfer (loading of embryos into embryo transfer catheter)
Advanced IVF techniques:
• Assisted hatching (laser)*
• Embryo biopsy *
• Oocyte Spindle imaging*
Andrology Techniques
• Semen Analysis (manual/CASA)
• Sperm preparation for IUI/IVF/ICSI
• Method of retrieval of sperm from retrograde ejaculate *
• Testicular sperm retrieval (mouse)
• Consent forms & documentation

Duration of hands on training:
• 14-days

Mode of teaching:
• Lectures
• Multimedia presentations
• Interactive discussions

Each participant will receive:
• Certificate of training
*Demonstration, mouse oocytes/sperm will be used.

Hands on training in cryopreservation techniques

During this course program each participant receives hands on training in the following cryopreservation techniques:
Human spermatozoa freezing:
-By vapour freezing method
- By Planar technique
- Isolation and cryopreservation of sperm from testicular biopsy specimens*
Vitrification:
Embryos
Blastocysts
Preparation of vitrification media
Consent forms & documentation

* Demonstration
Mode of teaching:
• Multimedia presentation
• Interactive discussions

Each participant will receive:
• Course manual
• Certificate of training

Duration of hands on training:
• 4-days