Rama Raju was born in Guntur, Guntur district in the Indian state of Andhra Pradesh. son of Gottumukkala Rama Krishnam Raju and Gottumukkala Venkata Narasamma. He completed his schooling in Gandhi Centenary School, Kakinada (1975). Later he pursed Intermediate in Bi.P.C. stream in DNR College Bhimavaram (May, 1977). He obtained his bachelor’s degree in Medical Sciences from Andhra Medical Sciences, Visakhapatnam (May 1986).
Rama Raju graduated with a medical degree and completed post-graduate work in 1990 at Andhra Medical College and National Board of Examination India. He studied ultrasound at Wade Maternity Hospital Mumbai.