Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging.
Rama Raju GA, Prakash GJ, Krishna KM, Madan K.
This study assesses meiotic spindle and zona pellucida characteristics using the PolScope, and analyses their relationship to embryonic development potential. A total of 205 matured oocytes retrieved from 25 patients undergoing ovarian stimulation were imaged for meiotic spindle and zona pellucida characteristics using the PolScope. After intracytoplasmic sperm injection, the oocytes were cultured and assessed for progression to blastocysts. Meiotic spindles were visualized in 78.0% of oocytes. Significantly more oocytes with visible spindles fertilized and progressed to blastocysts compared with oocytes without visible spindles. Oocytes with spindle retardance of >3 nm showed a greater progression to blastocysts compared with those with a retardance of 2-3 nm or 12 nm than from oocytes with spindle lengths 10-12 nm or 3 nm compared with oocytes with retardance of 2-3 nm or
Reference by: https://www.ncbi.nlm.nih.gov/pubmed/17298718